A Digital Micromirror Device (DMD)-based Multi-Object Spectrograph (D-MOS) with an integrated imager has been developed. The optical performance of the MOS is evaluated through comprehensive laboratory calibration and on-sky observations using the 1.3-meter J.C. Bhattacharya (JCB) Telescope at the Vainu Bappu Observatory (VBO). The system is designed to assess the viability of using a DMD as a programmable slit mechanism for future ultraviolet-optical space missions. A complete imager-cum-spectrograph assembly was constructed using off-the-shelf optical components and configured for operation in the optical band, employing a DLP9500 DMD with a 1920×1080 micromirror array. Calibration experiments established the DMD-to-detector coordinate mapping and validated the strategies for object selection and slit placement. On-sky tests in crowded stellar fields confirmed successful slit targeting, precise object alignment, and multiplexed spectral acquisition. The spectrograph achieved a peak efficiency of 32%, a spectral resolving power of R∼1000 at 6000Å, a multiplexing capability of up to 46 slits (extendable to 85), and a contrast ratio of ∼ 6000. These results demonstrate the robustness and effectiveness of the DMD MOS system under real observational conditions and raise its TRL level for use in next-generation spectroscopic space missions.

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